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Probe : WMS577

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Locus Xgwm577-7B
      Rht13
      Xgwm577    
Reference A microsatellite map of wheat
          Microsatellite variation in Avena sterilis oat germplasm 
General_remark Insert contains repeat (CA)14(TA)6                                                                                   
               PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY.                                                               
                 REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.                                                       
               In 369 Avena sterilis accessions there were 2 alleles of                                                             
                 size 50-55 bp, PIC = negative.                                                                                       
Type Genomic 
     PCR 
     SSR   
PCR_primers 5' ATGGCATAATTTGGTGAAATTG 3' 
            5' TGTTTCAAGCCCAACTTCTATT 3'   
Amplification_conditions 3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min                                                                                      
                           at 55 deg C, and 2 min at 72 deg C; and a final extension                                                                                     
                           step of 10 min at 72 deg C                                                                                                                    
Polymorphism WMS577
Origin Source_species Triticum aestivum
       Source_germplasm Chinese Spring 
Data_source Roeder, Marion S. 98.09.15
            Fu, Yong-Bi       2007.04  

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