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Locus Xgwm499-5B
Xgwm499
Reference A microsatellite map of wheat
General_remark Insert contains repeat (GA)32
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY.
REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type PCR
SSR
PCR_primers 5' ACTTGTATGCTCCATTGATTGG 3'
5' GGGGAGTGGAAACTGCATAA 3'
Amplification_conditions 3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min
at 60 deg C, and 2 min at 72 deg C; and a final extension
step of 10 min at 72 deg C
Polymorphism WMS499
Origin Source_species Triticum aestivum
Source_germplasm Chinese Spring
Data_source Roeder, Marion S. 98.09.15
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