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Probe : WMS44

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Locus Xgwm44-7D
      Xgwm44
      Xgwm44-4A
      Stb5      
Reference A microsatellite map of wheat
          Abundance, variability and chromosomal location of
            microsatellites in wheat.
          Application of microsatellite markers to distinguish
            inter-varietal chromosome substitution lines of wheat
            (Triticum aestivum L.).                                                                                                          
General_remark Insert contains repeat (GA)28                                                                                        
               PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY.                                                               
                 REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.                                                       
               Repeat type (GA)x28.                                                                                                 
               Product size 182 bp in Chinese Spring.                                                                                 
Type PCR 
     SSR   
PCR_primers 5' GTTGAGCTTTTCAGTTCGGC 3' 
            5' ACTGGCATCCACTGAGCTG 3'    
Amplification_conditions 3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min                                                                                      
                           at 60 deg C, and 2 min at 72 deg C; and a final extension                                                                                     
                           step of 10 min at 72 deg C                                                                                                                    
Polymorphism WMS44
Origin Source_species Triticum aestivum
       Source_germplasm Chinese Spring 
Data_source Roeder, Marion S. 98.09.15
                              94.10.17   
            Korzun, Victor N. 97.10    

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