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Probe : WMS247

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Locus Xgwm247-3B
      Xgwm247-3A
      Xgwm247
      Xgwm247-6R
      Xgwm247.1-1B 
Reference A microsatellite map of wheat
          Microsatellite variation in Avena sterilis oat germplasm 
General_remark Insert contains repeat (GA)24                                                                                        
               PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY.                                                               
                 REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.                                                       
               In 369 Avena sterilis accessions there were 4 alleles of                                                             
                 size 39-60 bp, PIC = 0.04.                                                                                           
Type Genomic 
     PCR 
     SSR   
PCR_primers 5' GCAATCTTTTTTCTGACCACG 3' 
            5' ATGTGCATGTCGGACGC 3'       
Amplification_conditions 3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min                                                                                      
                           at 55 deg C, and 2 min at 72 deg C; and a final extension                                                                                     
                           step of 10 min at 72 deg C                                                                                                                    
Polymorphism WMS247
Origin Source_species Triticum aestivum
       Source_germplasm Chinese Spring 
Data_source Roeder, Marion S. 98.09.15
            Fu, Yong-Bi       2007.04  

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