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Probe : WMC313

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Locus Xwmc313-4A
      Xwmc313
      Stb7       
Reference SSRs from the Wheat Microsatellite Consortium
General_remark Wheat Microsatellite Consortium Project 1, Plate reference                                           
                 14f2                                                                                               
               Donor: Hjortsholm, Sejet                                                                             
               Location from nullisomic/tetrasomic (Daryl Somers,                                                   
                 AgCanada,  Feb 2001) : 198  bases located on  4A                                                   
               Trimmed to Rsa1 at both ends.  (CA)18 166 to 201.                                                      
Type PCR 
     SSR   
PCR_primers Forward gcAgTcTAATTATcTgcTggcg at 78 length 22 bases, Tm 63  
            Reverse gggTccTTgTcTAcTcATgTcT at 253 length 22 bases, Tm 59   
SSR_size 197 bases expected, Chinese Spring 
Amplification_conditions 51C anneal, polymorphic product on agarose; 51C anneal,                                            
                           polymorphic on acrylamide  (several loci).                                                       
Sequence WMC313
Origin Source_species Triticum aestivum
Data_source Somers, Daryl J. 2004.06
            Isaac, Peter G.  2004.06
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