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Probe : WMC25

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Locus Xwmc25b-2B
      Xwmc25a-2D
      Xwmc25.1
      Xwmc25.2
      Xwmc25y
      Xwmc25-2D
      Xwmc25-2B  
Reference SSRs from the Wheat Microsatellite Consortium
General_remark Wheat Microsatellite Consortium Project 1, Plate reference     
                 2c9                                                          
               Donor: Wang, Utah                                              
               (GT)26 193 to 244.  >2 Polymorphic loci.                         
Type PCR 
     SSR   
PCR_primers Forward TcTggccAggATcAATATTAcT at 102 length 22 bases, Tm      
              59.0                                                         
            Reverse TAAgATAcATAgATccAAcAcc at 246 length 22 bases, Tm      
              53.0                                                           
SSR_size 166 bases expected, Chinese Spring 
Amplification_conditions 51C anneal, polymorphic product on agarose; 51C anneal,                             
                           polymorphic on acrylamide.;                                                       
Sequence WMC25
Origin Source_species Triticum aestivum
Data_source Somers, Daryl J. 2004.06
            Isaac, Peter G.  2004.06 

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