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Probe : CDO583

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Locus cdo583
      Xcnl.CDO583
      Xcdo583-3B
      Xcdo583a
      Xcdo583b    
Reference Construction of a restriction fragment length polymorphism
            map for barley (Hordeum vulgare)
General_remark Uni-ZAP XR library 
               OatUni-ZAPXR [c]     
Type cDNA 
Sequence BE439192
         CDO583_WHE03A04.r1 
Polymorphism CDO583 XbaI
             CDO583 EcoRI
             CDO583 EcoRV
             CDO583 HindIII
             CDO583 DraI    
Linkage_Group [b 7L] 
Origin Insert_enzyme EcoRI XhoI
       Source_species Avena sativa
       Source_germplasm Brooks CIav9260
       Source_tissue etiolated leaf   
Insert Size 1.7 
       PCR_size 1.7   
Clone Vector pBluSK 
      Excision_enzyme EcoRI XhoI
      Vector_PCR_primers M13forward 
                         M13reverse   
      Vector_amplification 94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C                                              
                             for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time                                                         
      Antibiotic Ampicillin 
      Location Sorrells, Mark E.
               GrainGenes Probe Repository 
      Authority Sorrells, Mark E. 
Image CDO583 SO autoradiogram
Data_source Sorrells, Mark E. 96.07
            ATMI              92.09
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