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DNA BE403741 522
Contig Ta.2739.1.S1_x_at
NSFT03P2_Contig18731
Tracefile A/tracefiles/Run_ARS3700_2000-04-27_183/0436_B08_D16Z_061.a\ [download|view]
b1
DB_info External_DB GenBank BE403741 [DDBJ|EMBL|GenBank]
TIGR_TC TC278991 [TIGR]
UniGene Ta.54243 [NCBI]
DB_remark Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Ribosomal protein L19'
Keyword EST
Origin Germplasm Chinese Spring
Species Triticum aestivum
Cultivar Chinese Spring
Clone_lib Wheat etiolated seedling root cDNA library
Tissue Root
Dev_stage Five day old etiolated seedling
Date 00-07-21_01
Data_source genbank Release 135, Apr 15 2003
Updated Nov 2006
Visible Title WHE0436_B08_D16ZS Wheat etiolated seedling root cDNA library
Triticum aestivum cDNA clone WHE0436_B08_D16, mRNA sequence.
Other_name WHE0436_B08_D16ZS [wEST|wEST-SQL]
Strain lab_host E. coli SOLR
DNA_library TA008E1X
Clone WHE0436_B08_D16
Remark Locus Comment: Contact: Olin Anderson; US Department of
Agriculture, Agriculture Research Service, Pacific; West
Area, Western Regional Research Center; 800 Buchanan Street,
Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818;
Email: oandersn@pw.usda.gov; Sequence have been trimmed to
remove vector sequence and low; quality sequence with phred
score less than 20; Seq primer: Strategene SK primer.
DB_xref: taxon:4565
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1:
EcoRI; Site_2: XhoI; Seeds were surface-sterilized ,
germinated and grown aseptically in the dark at room
temperature on filter paper with water, nystatin and
cefotaxime in covered crystallization dishes. Roots were
harvested. The tissue, total RNA, and poly(A) RNA were
prepared, a cDNA library was made, and the cDNA clones were
in vivo excised to give pBluescript phagemids in the TJ
Close lab (Choi, Close, Fenton) at the University of
California, Riverside. Plasmid DNA preparations and DNA
sequencing were performed in the OD Anderson lab (all other
authors).
Feature: source:
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1:
EcoRI; Site_2: XhoI; Seeds were surface-sterilized,
germinated and grown aseptically in the dark at room
temperature on filter paper with water, nystatin and
cefotaxime in covered crystallization dishes. Roots were
harvested. The tissue, total RNA, and poly(A) RNA were
prepared, a cDNA library was made, and the cDNA clones were
in vivo excised to give pBluescript phagemids in the TJ
Close lab (Choi, Close, Fenton) at the University of
California, Riverside. Plasmid DNA preparations and DNA
sequencing were performed in the OD Anderson lab (all other
authors).
Feature: source: mol_type = 'mRNA';
Properties RNA
Transcript mRNA
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