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Probe : BCD926

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Locus Xbcd926-5A
      bcd926
      Xbcd926
      Xbcd926-5D
      Xbcd926-5B 
Reference Construction of a restriction fragment length polymorphism
            map for barley (Hordeum vulgare)
General_remark Lambda ZAP II library 
               Bar.LambZAPII [c]       
Type cDNA 
Polymorphism BCD926 EcoRI
             Durums BCD926 EcoRI
             BCD926 HindIII      
Linkage_Group 5L 
Origin Insert_enzyme EcoRI
       Source_species Hordeum vulgare
       Source_germplasm Willis
       Source_tissue etiolated leaf   
Insert Size 1.4 
       PCR_size 1.4   
Clone Vector pBluSK  
             pBluSK-   
      Excision_enzyme EcoRI
      Vector_PCR_primers M13forward 
                         M13reverse   
      Vector_amplification 94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C                                             
                             for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time                                                          
      Antibiotic Ampicillin 
      Location Sorrells, Mark E.
      Authority Sorrells, Mark E. 
Image BCD926 BROWS autoradiogram
      BCD926 SO autoradiogram    
Data_source Sorrells, Mark E. 96.07
            ATMI              92.09
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