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STS PCR_primers STS_primers Amplification_conditions
5S rDNA PI/PIIPI, 5' tgcaacac(a/g)aggacttccca-Reaction mixture in 25 ul volume containing 50 mM KCl, 1.5 mM MgCl2, 10 mM Tris.HCl (pH 8.3), 0.2 mM each of dATP, dTTP, dGTP, and dCTP, 0.4 uM each primer, 2 units of Stoffel fragment, and 2.5 ul of template. PCR amplification performed at 95oC for 5 min, 30 cycles of 94oC for 55 s, 60oC for 1 s, and 72o C for 40 s, and final extension at 72oC for 5 min.
5S rDNA PI/PIIPII, 5' (t/g)t(a/c)g(t/c)gctggtatgatcgca-Reaction mixture in 25 ul volume containing 50 mM KCl, 1.5 mM MgCl2, 10 mM Tris.HCl (pH 8.3), 0.2 mM each of dATP, dTTP, dGTP, and dCTP, 0.4 uM each primer, 2 units of Stoffel fragment, and 2.5 ul of template. PCR amplification performed at 95oC for 5 min, 30 cycles of 94oC for 55 s, 60oC for 1 s, and 72o C for 40 s, and final extension at 72oC for 5 min.
0090SForward CCTACCGCTCCATAGTTGGTATCTA--
0090SReverse ACACACACACACACACACATATATA--
270-1F/1R1F 5' GCAATTTAATCAAGCATCCAAAATTCATG-55
270-1F/1R1R 5' CGAGAAACAGCAAGAACACCACCAAC-55
270S-1F/1R1F 5' ATTCTTGTTGGTGGTCGCTTGGTG-55
270S-1F/1R1R 5' AACTGCTACACCCACATACCCACAGG-55
669-1F/1R1F 5' GCTTTCCCTTTGCTAGAGCTTGGA-55
669-1F/1R1R 5' TCCTGCTGGAAAACATGGCACA-55
1158-1F/1R1F 5' GCTCTTGGTACAGCAAAAGCCTTAGAGTA-55
1158-1F/1R1R 5' TCTCCACGGAATAGAAGGATCAACCA-55
1407-1F/1R1F 5' GCAGCATATTTGGAAACAACTTAAGAAACAC-55
1407-1F/1R1R 5' CTTCGAGCTGTTGCTTCCAATGAG-55
1882-1F/1R1F 5' CAACACCAGTTCAAGATTACCCTATGAAGA-55
1882-1F/1R1R 5' TGCTCCTGATCTTAAGGTCCATAAAGTCG-55
1950-2F/2R2F 5' CCACCTACGTCGTCGGCGTCAATG-55
1950-2F/2R2F 5' GTCGCGGAACGCCTGGTTCACCTC-55
AB009308Forward ATGGAATCTTATCGCTGTTC--
AB009308Reverse CGGCAGATTTGAGAACTATTA--
ABA1CACGCCCTCGCCAACGCTCTCCA--
ABA1GGGGAGATATCGACCAAAGT--
ABA2GGCAGCAACATGGCATTC--
ABA2CTGGGGTTGGTTCTGG--
ABA3GACCTCCACGAGTTGC--
ABA3GCTGCGCGCTTCAGCT--
ABA4GGGTGGCGTGGGGTG--
ABA4CCACCAAGCGTGGAGTC--
ABA5L 5' GGT ACG AAC ATG GAG GTA CT 3'--
ABA5R 5' ATC CAG TTC TTG TGC ACC TG 3'--
ABA65'GAG GAA GGT CTT CAT GGT GGC3'--
ABA301L 5'-TCC GAA CGT TTT GTA ACC CCG GG-3'--
ABA301R telomere primer (see ABA303)--
ABA3035'-CCG AAT TCA ACC CTA AAC CCT AAA CCC TAA ACC C-3'--
ABA307Tel81L - 5'CGG TTT TAG CGT TGG CCA TGG TAT3'--
ABA307Tel R - 5'CCG AAT TCA ACC CTA AAC CCT AAA CCC TAA ACC3'--
ABC00046_L01R015' GAGCACGAGGATGAGAGGAG--
ABC00046_L01R015' TTTTACGGGATCAAAGCTGAG--
ABC00103_L01R015' GGTAAGGAGTGGGTCTCAGG--
ABC00103_L01R015' CAAGCAGATGCAACTACACC--
ABC00149_L01R015' TTGTGGGGTTTTACCTGTGA--
ABC00149_L01R015' ACAAACCAACCGAGCAGAAT--
ABC151GTG CCG TAG ATA GGA AGG TG--
ABC151CAT GGT ACA AAC TCT CAA CT--
ABC152TCC GCA AGT ACC AGA AGA GC5' AGTCGGCGCCGACCACCGGCGG; 5' AGTCGGCGCCGACCACCGGCGGSTS annealing temperature 58-60 oC
ABC152GAC AAG GAA AGC CAA TCA AC5' AGTCGGCGCCGACCACCGGCGG; 5' AGTCGGCGCCGACCACCGGCGGSTS annealing temperature 58-60 oC
ABC153GCC TCT GCC GCT GGA ACT AC5' GGTTTGAGGACATGGTTGTTTC; 5' GCCTCTGCCGCTGGAACTACSTS annealing temperature 52-55 oC
ABC153AAA CAC CTC CTG GCT CTC AG5' GGTTTGAGGACATGGTTGTTTC; 5' GCCTCTGCCGCTGGAACTACSTS annealing temperature 52-55 oC
ABC154TTC ATT TCA TCG TTT TCA TA--
ABC154ATC AGG TTC AGC AGT GTT TA--
ABC155TCT GTG TGT TAT TCC TCT TA--
ABC155ACG AAA AAT CAA TAC TGG TC--
ABC156-5' TTACGGGATCAAAGCTGAGGC; 5' GACAAGCAACACCAACCAAGCSTS annealing temperature 58-60 oC
ABC156.1GAG AGG AGG ACA AGC AAC AC--
ABC156.1ATG AGG AAG GAC ACA CTG GT--
ABC156.2GAATATGAAGATTCTGATCT--
ABC156.2TCATCATCCAAGAACGAAGG--
ABC156.3GAGAGGAGGACAAGCAACAC--
ABC156.3TCATCATCCAAGAACGAAGG--
ABC156.4CCGGCAGAGGCAGCACGAGG--
ABC156.4TCATCATCCAAGAACGAAGG--
ABC156.5CAAGGAGGATCAAGTAGTCA--
ABC156.5CATAATGTGGGGTGCCATAC--
ABC158AAC AGA GGA CAA CGA TAC AC--
ABC158AAA GCT AGG ACA AAA ATA CC--
ABC160GCCAGCGCTCCGGTCTGGTC--
ABC160AACAAGGCCGCATGGATGTA--
ABC161TTG TCA AAC ATG GAT TGT GT--
ABC161CGC CTC GGG GTC AAG ATT TA--
ABC163-5' TTACGGGATCAAAGCTGAGGC; 5' TGGCAGCGCTGTTGTAGGTTSTS annealing temperature 55-58 oC
ABC164CGC ACA CCT CTC CCT CCT TC5' CAAGAGCACAGGATGGCGACCGTCC; 5' GAGGCAGCCCTGATGTAGTACCAGTSTS annealing temperature 52-55 oC
ABC164ATG CCC ATC TTC TGC AAC TC5' CAAGAGCACAGGATGGCGACCGTCC; 5' GAGGCAGCCCTGATGTAGTACCAGTSTS annealing temperature 52-55 oC
ABC165CAATGACTTCAAGGGGTCTG--
ABC165TCCATACCATTCCCATCTAA--
ABC166GTC CGA GGG CCG CCC TAC AC--
ABC166CCT GAA TCA ACA ACC AAA TA--
ABC167TGG CTG GCG GAA TGG AAT AA5' CAGCCCATGAGCTAGTGTTCC; 5' TGGATGATGAAATTCCAGAGGSTS annealing temperature 52-55 oC
ABC167ACA AGT TTG GAT GAT GAA AT5' CAGCCCATGAGCTAGTGTTCC; 5' TGGATGATGAAATTCCAGAGGSTS annealing temperature 52-55 oC
ABC168GTA AGA ACA AAA CCC AAA AC5' CCCGGTAACTACTTACACCG; 5' CGTGGAGCTGGTGTGCGAGASTS annealing temperature 55-58 oC
ABC168GGA GAC CCA GCG GAA GGA GA5' CCCGGTAACTACTTACACCG; 5' CGTGGAGCTGGTGTGCGAGASTS annealing temperature 55-58 oC
ABC170ACG GTT GCT GTG GGT GTG AT--
ABC170AAC GAT ACA ATG ACC AGA GC--
ABC175-5' CAGTTTACAAGTGCAGAGGT; 5' GAACAGAAGCAGAAGATCGASTS annealing temperature 55-58 oC
ABC00177_L02R025' AATCTGCTCATCCCATCTGC--
ABC00177_L02R025' GTTCAGGCAATGCAGCATAA--
ABC252CACAAGGCTCAAAACATAAC--
ABC252AAGCTCACCAAGTCCCAGTC--
ABC253AGGGAATGCAGATCTCACAC--
ABC253GCATGGTGACAGATTTCAAA--
ABC254CTCACCACCGAGGCCAAGAA--
ABC254AAGAAAGCTGAAGGCACATT--
ABC255GATCATCGTGGATGGAGTGT--
ABC255CGAATTCCGAGACATCAAAT--
ABC257TTCAACGAAAACTCTGTCCA--
ABC257GACGCCAGAGCTAGCAAAGT--
ABC261AGGAAGCTCAAGAAGGTGAA--
ABC261AAAGTCAAGAGTTGCATCAA--
ABC302-5' CTGGTGCTTTCGATGCTGT; 5' CTGAGTCTTCGCCATGCATASTS annealing temperature 58-60 oC
ABC302.1CAGAGTACAGGACAGACAGT--
ABC302.1GTGGTAAAGGAGCGGTTGAT--
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